Important Notice

Important Notice: Due to the merger of Tokyo Tech and TMDU, this site will be closed on September 30, 2024.
Please refer to the Alumni Association Web page or Prof. Wakabayashi Web page from now on.

The Hisabori & Wakabayashi Laboratory has terminated its activities as of March 31, 2023, following the retirement of Professor Hisabori.
Therefore, no new students or research students will be accepted. In addition, with the termination of the laboratory's activities, this Web page will no longer be updated as of March 31, 2023. We have opened a new Alumni Association Web page , and new information will be posted here from now on. Thank you for your cooperation.

News & Topics

2023.1.31
A new paper on the study of the redox regulation apparatus of CFoCF1 by Mr. Kentaro Akiyama et al. has been published in Proc. Natl. Acad. Sci. USA .
2023.1.5
A new paper on the metabolie analyses of the lipid synthesis mutated cyanobacteria by Assoc. Prof. Kumiko Kondo et al. has been published in Scientific Reports .
2022.10.28
A new paper on the importance of the ferredoxin/thioredoxin pathway in chloroplasts by Assoc. Prof. Keisuke Yoshida together with our group has been published in Journal of Biological Chemistry .
2022.9.27
A new paper on the oxidation of the chloroplast ATP synthase by Mr. Takatoshi Sekiguchi et al. has been published in Journal of Biological Chemistry .
2022.6.22
A short lecture by Prof. Toru Hisabori at the 8th meeting of the Specialized Academy for Cell Science, Organization for Fundamental Research, Tokyo Tech. is reported.
2022.4.13
A new paper on the verification of Trx-target protein localization by Ms. Yuka Fukushi et al. has been published in Antioxidants .
2022.4.13
A new paper on the regulation mechanism of phosphoribulokinase in photosynthetic organisms by Ms. Kazuha Fukui, Dr. Shoko Mihara et al. has been published in Plant and Cell Physiology .
2022.2.7
A new paper on the mammalian-type thioredoxin reductase in Chlamydomonas by Mr. Yuma Asahina and Mr. Kazuma Sakamoto has been published in Biochem Biophys Res Commun .
2021.12.21
A new paper on the physiological function of oxidation factors in chloroplasts by Dr. Yuichi Yokochi and Ms. Yuka Fukushi has been published in Proc. Natl. Acad. Sci. USA .
2021.9.10
A new paper on the new redox sensor protein ROBIN by Ms. Nae Fu has been published in Journal of Biological Chemistry .
2021.8.3
A new paper on the phototroph-specific beta-hairpin structure of the gamma subunit of ATP synthase by Dr. Kumiko Kondo has been published in Journal of Biological Chemistry .
2021.7.20
A new paper entitled 'Characterization of Chlamydomonas reinhardtii Mutants That Exhibit Strong Positive Phototaxis' by Mr. Jun Morishita has been published in Plants .
2021.2.4
A new paper on the cyanobacterial NTRC by Dr. Frederic Deschoenmaeker has been published in J. Biochem. (Tokyo) .
2021.2.3
A new paper on the redox regulation mutant of malate dehydrogenase of A. thaliana by Dr. Yuichi Yokochi has been published in Proc. Natl. Acal. Sci. USA .
2021.1.9
A new paper on the redox regulation of phosphofructokinase from A. thaliana by Dr. Keisuke Yoshida has been published in Plant Cell Physiology .
2021.1.6
A new book Advances in Experimental Medicine and Biology, Optogenetics 2nd ed. contributed by Prof. Ken-ichi Wakabayashi has been published.
2020.12.3
A new paper on the analysis of intracellular ATP by using flagellar movement of Chlamydomonas reinhardtii by Ms. Wakako Takano has been published in Journal of Biological Chemistry .
2020.12.1
A new paper on the luminescent pH sensor protein by Mr. Shungo Nakamura has been published in Journal of Biological Chemistry .
2020.11.22
Our Assistant Professor Jiro Nomata passed away due to bile duct cancer. We pray that he may rest in peace.
2020.8.26
A new paper on the redox regulation of plastidial 3-phosphoglycerate dehydrogenase by Dr. Keisuke Yoshida has been published in J. Biol. Chem. .
2020.7.11
A new paper on the kinetic analysis of chloroplast ATP synthase reduction by Mr. Takatoshi Sekiguchi has been published in Biochim. Biophys. Acta - Bioenergetics .
2020.6.23
A new paper on FROG/B, a new redox sensor protein by Dr. Kazunori Sugiura has been published in Proc. Natl. Acal. Sci. USA .
2019.12.21
A new paper on the analysis of the thioredoxin target proteins in Heterocyst of cyanobacteria Anabaena by Dr. Shoko Mihara has been published in J. Exp. Bot. .
2019.10.9
A new paper on the key residues of thioredoxin-f by the graduate course student Yuichi Yokochi has been published in J. Biol. Chem. .
2019.10.1
A new paper on the analysis of the dynein docking complex in planaria cilia by the graduate course student Ayaka Kyuji has been published in Zoological Science .
2019.6.20
A new paper on the thioredoxin redox sensor protein CROST by Dr. Kazunori Sugiura has been published in J. Biol. Chem. .
2019.6.7
A new paper on TrxL2 mediated oxidative activation of the chloroplast G6PDH in Arabidopsis thaliana by Dr. Keisuke Yoshida has been published in Biochemical Journal .
2019.6.5
A new paper on the simple method to determine protein redox state in Arabidopsis thaliana by Dr. Keisuke Yoshida has been published in bio-protocol .
2019.6.5
A new paper on the gamma subunit of cyanobacterial ATP synthase by Mr. Kentaro Akiyama has been published in Biochemical Journal .
2019.5.10
A new paper on the epsilon subunit of cyanobacterial ATP synthase by Mr. Kosuke Inabe has been published in Journal of Biological Chemistry .
2019.4.8
A new paper on the proteome analysis of thioredoxin defficient mutant of cyanobacteria Part II by Dr. Deschoenmaeker Frederic has been published in Plant and Cell Physiology .
2019.4.5
A new paper on the new redox sensor protein Re-Q by Dr. Kazunori Sugiura has been published in Biochim Biophys Acta - General Subjects .
2019.3.11
A new paper on the analysis of the inner lumen proteins in cilia and flagella co-authored by Prof. Yoshikawa's group at Univ. Tokyo and Dr. Ken-ichi Wakabayashi has been published in Nature Communications .
2018.11.1
A new paper on the analysis of the rate limiting step of redox regulation in chloroplasts by Dr. Keisuke Yoshida has been published in Antioxidants .
2018.8.13
A new paper on the oxidation factor in chloroplasts by Dr. Keisuke Yoshida has been published in Proc. Natl. Acad. Sci. USA .
2018.8.10
A new paper on the assessment of the flagellar redox potential in Chlamydomonas reinhardtii by Dr. Kazunori Sugiura, Mr. Yuta Nishimaki and Dr. Ken-ichi Wakabayashi has been published in Biochem. Biophys. Res. Commun. .
2018.8.9
A new paper on the proteome analysis of thioredoxin defficient mutant of cyanobacteria by Dr. Deschoenmaeker Frederic has been published in Plant and Cell Physiology .
2018.8.7
A new paper on the novel oxygen sensor protein by Dr. Jiro Nomata has been published in Scientific Reports .
2018.7.27
A new paper on the crystal structure analysis of the gamma-epsilon subcomplex of F1-ATPase obtained from the cyanobacterium Thermosynecococcus elongatus BP-1 by Prof. Satoshi Murakami, Dr. Kumiko Kondo, and Mr. Shinya Katayama et al. has been published in Biochemical Journal .
2018.2.19
A new paper on the analysis of the property of the gamma subunit of F1-ATPase obtained from the cyanobacterium Thermosynecococcus elongatus BP-1 by Dr. Kumiko Kondo has been published in Biochim. Biophys. Acta .
2018.2.13
A new paper on the analysis of the redox regulation mechanism of G6PDH of the cyanobacterium Anabaena sp. PCC7120 by Ms. Shoko Mihara has been published in Biochemical Journal .
2018.1.30
A new paper on the analysis of the flagellar Ca2+ sensitivity of Volvox by Dr. Noriko Ueki and Dr. Ken-ichi Wakabayashi has been published in Proc. Natl. Acad. Sci USA .
2017.11.07
A new paper on the application of CRISPR interference for metabolic engineering of the cyanobacterium Anabaena sp. PCC7120 by Dr. Akiyoshi Higo has been published in Plant and Cell Physiology .
2017.10.23
A new paper on the newly designed gene expression system in the cyanobacterium Anabaena sp. PCC7120 by Dr. Akiyoshi Higo has been published in Plant and Cell Physiology .
2017.9.13
A new paper on the analysis of ROS torerance of ATP synthase of Chlamydomonas reinhardtii by Dr. Felix Buchert, our former PD, has been published in Biochim. Biophys. Acta .
2017.3.1
A new paper on the analysis of thioredoxin network in Arabidopsis by Dr. Keisuke Yoshida has been published in Biochemical Journal .
2016.12.24
A new paper on the analysis of NTRC and 2-Cys Prx in the cyanobacterium Anabaena sp. PCC7120 by Ms. Shoko Mihara has been published in Plant and Cell Physiology .
2016.11.24
A new paper on the analysis of the motility diversity of volvox species by Dr. Noriko Ueki has been published in PLoS ONE .
2016.9.23
A new paper on the newly designed gene expression network in the cyanobacterium Anabaena sp. PCC7120 by Dr. Akiyoshi Higo has been published in ACS Synthetic Biology .
2016.6.23
A new paper on the finding of the novel redox regulation pathway in plant chloroplast via NTRC by Dr. Keisuke Yoshida has been published in Proc. Natl. Acad. Sci. USA .
2016.4.28
A new paper entitled "Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii" by Dr. Noriko Ueki, Dr. Takahiro Ide, and Dr. Ken-ichi Wakabayashi has been published in Proc. Natl. Acad. Sci. USA .
2016.3.2
A new paper on the finding of the novel regulation mechanism of plant mitochondria MDH by Dr. Keisuke Yoshida has been published in Biochim. Biophys. Acta - Bioenergetics .
2015.12.18
A new paper on the metabolic engineering of cyanobacteria Anabaena sp. PCC7120 by Dr. Akiyoshi Higo has been published in Plant Cell Physiolosy
2015.5.6
A new paper on the analysis of the thioredoxin target proteins in cyanobacteria Anabaena sp. PCC7120 by collaboration of Dr. Jiro Nomana and Ms. Maki Maeda has been published in J. Biochem. .
2015.4.18
A new group photo was uploaded at "Members" page.
2015.4.16
A new paper on the analysis of the specificity of Arabidopsis thioredoxins by Dr. Keisuke Yoshida has been published in J. Biol. Chem. .
2015.3.14
A new paper on the method to analyse the motion of Chlamydomonas flagella by Assoc. Prof. Wakabayashi has been published in Methods in Cell Biology .
2015.2.9
A new paper on the analysis of the regulation mechanism on the redox-sensitive ATP synthase by Felix Buchert has been published in Biochim. Biophys. Acta - Bioenergetics .
2015.1.14
A new paper on the development of the useful redox-sensitive fluorescence protein by Kazunori Sugiura has been published in Biochem. Biophys. Res. Commun. .
2014.11.24
A new paper on the development of the useful thiol detection compounds, DNA-PCmaleimide by Dr. Satoshi Hara has been published in Biochem. Biophys. Res. Commun. .
2014.9.24
A new paper on redox regulation of respiratory enzyme in plant mitochondria by Dr. Keisuke Yoshida has been published in frontiers in ENVIRONMENTAL SCIENCE .
2014.7.1
A new paper on the cooperative binding of the outer arm-docking complex of outer arm dynein by Dr. Mikito Owa has been published on-line in Proc. Natl. Acad. Sci. USA .
2014.5.20
A new paper on the dynamics of thiol enzymes in chloroplasts by Dr. Keisuke Yoshida has been published on-line in Plant and Cell Physiology .
2014.4.8
A new group photo was uploaded at "Members page".
2014.3.5
A new paper on the photochromic regulation of F1-ATPase using ATP-Azo by Dr. Sunamura as a co-work with Tamaoki Lab. at RIES, Hokkaido Univ. has been published on-line in Biochem. Biophys. Res. Commun. .
2013.12.19
A new paper on the kinetic analysis of the protein-protein interaction between thioredoxin and its target proteins by Dr. Hara has been published on-line in Frontiers in Plant Science .
2013.12.16
Dr. Kumiko Kondo-Osanai was assigned as a researcher on CREST project.
2013.6.12
A new paper on the protein-protein interactions between intermediate chains and the docking complex of Chlamydomonas flagellar outer arm dynein by Dr. Ide, the co-worker of our Assoc. Prof. Wakabayashi, has been published on-line in FEBS Letters .
2013.4.25
A new paper on the thioredoxin regulation of Calcium Dependent Protein Kinases in Plasma Membranes by Dr. Hanayo Ueoka-Nakanishi has been published on-line in FEBS Journal .
2013.4.1
Dr. Ei-Ichiro Sunamura was assigned as a researcher on CREST project.
2013.2.27
A new paper on the analysis of thioredoxin target proteins in plant mitochondria by Dr. Keisuke Yoshida has been published on-line in Plant Cell Physiology .
2013.2.4
Our Web-site renewal open
2013.2.1
Dr. Higo, CREST-researcher, was assigned as an assistant professor at CRL.
2013.1
A new paper on DNA-maleimide by Dr. Hara has been published in BBA General Subject .
2012.12
A new paper on redox regulation of cyanobacterial PGK by Tsukamoto and Fukushima has been publised in Plant Cell Physiology .
2012.12
A new paper on the analysis of the active site of DyP by Toru Yoshida has been published in FEBS Letters .
2012.11
A new paper on the functional analysis of the gamma subunit of ATP synthase by Sunamura has been published in J Biol Chem .
2012.11.1
Dr. Ken-ichi Wakabayashi was assigned as an associate professor at CRL.
2012.04
Dr. Keisuke Yoshida was assigned as an assistant professor at CRL.
2012.03
Our associate professor Dr. Yasushi Sugano was assigned as Profesor at Japan Women's University.

About us

Our research group is mainly working on the metabolism and movement of photosynthetic organisms as follows:
(1) Molecular mechanisms of rotation and regulation of ATP synthase from photosynthetic organisms
(2) Biochemical analysis of thioredoxin and its target proteins in photosynthetic organisms
(3) Anti-oxidant stress system in photosynthetic organisms
(4) Motility mechanism and its regulation of eukaryotic flagella

We carried out CREST project (2011-2016) together with Prof. M. Hara in Frontier Research Center

Now, we are working on 'New Photosynthesis' project lead by Prof. J. Minagawa at NIBB (2016-2020).